The method of collection of immature oocytes can affect mitochondrial morphology and function
Follicle containing immature oocytes can be collected from ovaroan tissue by
Mechanical disaggregation
(use forceps and seisors
2. Enzyme digestion of the extra cellular matrix and connective tissue that the follicles embedded in
- collagenase – DNAse – Libarase – tryse
3. A combination of mitochondrial and enzyme disagrregation
Collect ovaries from naturally cycling mice culled in
routine anthiel hwse procedures
2. Dissect into rephiate small sections
3. Distribute to 4% paraformaldehyde fixation
H+E histology to quantity follicule
Mechanical disaggregation
Collagenase concentration 1, 2 & 3
Trypsin concentration 1, 2 & 3
Selected cllagenase concentration + selected hypsin concentration
sTo determine optomum time for each incubation
Mechanical disaggregation
(use forceps and seisors
2. Enzyme digestion of the extra cellular matrix and connective tissue that the follicles embedded in
- collagenase – DNAse – Libarase – tryse
3. A combination of mitochondrial and enzyme disagrregation
Collect ovaries from naturally cycling mice culled in
routine anthiel hwse procedures
2. Dissect into rephiate small sections
3. Distribute to 4% paraformaldehyde fixation
H+E histology to quantity follicule
Mechanical disaggregation
Collagenase concentration 1, 2 & 3
Trypsin concentration 1, 2 & 3
Selected cllagenase concentration + selected hypsin concentration
sTo determine optomum time for each incubation
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